论文编号:SW080论文字数:10084,页数:28
摘 要：本文主要阐述了应用高效液相色谱（HPLC）方法测定水产品中阿苯达唑（ABZ）及其两种主要代谢物砜（ABZSO2）、亚砜（ABZSO）残留量的研究。色谱条件是：色谱柱为ZORBAX-SB 5μm C18柱；流动相是酸化乙腈：水(25:75；v/v)；甲醇-磷酸缓冲液（75︰25；v/v），流速是1.0mL/min，柱温是30℃，选择的检测波长是295.4nm。采用甲醇：磷酸缓冲液=75：25定容系列标准液，阿苯达唑、阿苯达唑砜、阿苯达唑亚砜在0.1～10μg/mL范围内线性关系良好，加标浓度在100μg/kg～500μg/kg时，回收率分别ABZSO为83%～95%；ABZSO2为85%～97%；ABZ为70%～80%。研究结果显示，该方法的最低检测限、准确度和精密度均符合残留检测方法的要求。此检测分析方法处理样品快速、高效、准确，可作为水产品中阿苯达唑及其主要代谢物的有效检测方法。
关键词：水产品、高效液相色谱法、阿苯达唑及其代谢物
Determination of Albendazole and its metabolites in Aquatic products by HPLC
Abstract: The determination of Albendazole and its metabolites in aquatic products was established by using high performance liquid chromatography (Agilent-1100, USA) with a photodiode array detector detector (DAD, USA), a C18 column (ZORBAX-SB). The mobile phase was a mixture of acidified acetonitrile and water (25/75, v/v) and a mixture of methanol and phosphate buffer (75/25, v/v). 20 µl samples at room temperature injected onto the column through a 20 µl sample loop. Column temperature is 300C.The mobile phase rate was delivered at 1.0 ml/min. Detection wavelength: 295.4nm. In the range of 0. 1 - 10μg/ml, the results of Albendazole and its metabolite are linearly dependent. The mean extraction recoveries of Albendazole and its metabolites ranged from 80% to 90%. The method of determining the Albendazole and its metabolites in aquatic product was established by using reversed-phase HPLC, which had characters of simple and rapid operation, high yield and good reproducibility.
Keywords：Aquatic products, high performance liquid chromatography, Albendazole and its metabolites
目 录
中文摘要I
英文摘要II
目录III
1. 绪论1
1.1 前言1
1.2 阿苯达唑及其主要代谢物检测技术的国内外发展现状2
2. 实验部分5
2.1 试剂和材料5
2.2 仪器设备5
2.3 实验操作方法6
3. 结果与讨论8
3.1 样品前处理条件的选择8
3.2 色谱条件的选择8
3.3 精密度和准确度14
3.4 方法的灵敏度和检测限19
4．总结与展望20
致谢21
参考文献22
HPLC法测定水产品中阿苯达唑及其主要代谢物残留的研究......