论文编号:SP021论文字数:7870,页数:17
摘 要
将从土壤中分离出来的8株木霉菌（Trichodema spp.），与节瓜枯萎病原菌进行对峙培养，筛选出拮抗节瓜枯萎病菌镰刀菌的优良木霉菌T04-004菌株，该菌株对节瓜枯萎病菌的抑制率达90%以上。对生防菌木霉菌T04-004进行生物学特性测定，结果表明，在Richard培养液中，T04-004菌株孢子萌发需要一定的营养，其中以PD培养液最好，并且在PDA培养基上菌丝生长最好，产孢量最大。T04-004菌株孢子萌发，菌丝生长和产孢的温度范围为15℃～40℃；T04-004菌株孢子萌发，菌丝生长和产孢的pH值范围为2～10；光照对菌丝的生长影响不显著，但对产孢量的影响较大。
关键词：木霉菌 节瓜枯萎病原菌 拮抗作用 生物学特性
Biological control Effect of Trichodema on Fusarium oxysporum f.sp.benincasae
Abstract： 8 Trichodema were collated from the soil, carries on confronts the Fusarium oxysporum f.sp.benineasae, screens the fine Trichodema T04-004 that oppresses Fusarium oxysporum f.sp.benineasae, This strain to the Fusarium oxysporum f.sp.benineasae ’s suppression rate achieved above 90%. The biological characterizations of Trichoderma viride T04-004 were screened and identified. The results illustrated that the spore germination of T04-004 strain needs certain nutrition, and the PD nutrient fluid is the best. The strain T04-004 grows well in the PDA culture medium, including the hypha and spore production. The temperature range of spore sprouts, hypha growth and spore production is 15℃~40℃, and the range of pH-value is 2~10. The illumination is not remarkable influence to hypha growth, but significantly affect on the spore production.
Key words: Trichodema; Fusarium oxysporum f.sp.benincasae; antagonism; biological characteristic
目 录
1 前言1
2 材料与方法2
2．1 木霉菌的分离纯化2
2．1．1 木霉菌的分离2
2．1．2 木霉菌的纯化和保存2
2．2 拮抗镰刀菌的木霉菌筛选2
2．2．1 供试菌株和材料2
2．2．2 对峙培养2
2．3 木霉菌生物学测定3
2．3．1 菌种来源3
2．3．2 环境因子对分生孢子萌发的影响3
2．3．3 环境因子对菌丝生长和产孢的影响3
3 结果及分析4
3．1 木霉菌的分离纯化4
3．2 拮抗镰刀菌的木霉菌筛选4
3．3 木霉菌生物学测定6
3．3．1 环境因子对孢子萌发的影响6
3．3．2 环境因子对菌丝生长和产孢量的影响7
4 结论与讨论10
4．1 木霉菌的分离纯化10
4．2 拮抗镰刀菌的木霉菌筛选10
4．3 木霉菌生物学测定10
4．3．1 关于环境因子对孢子萌发的影响10
4．3．2 关于环境因子对木霉菌生长速度和产孢量的影响11
参 考 文 献12
英 文 摘 要13
致 谢14
木霉菌对节瓜枯萎病的生物防治作用......