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婴幼儿谷基配方米粉酶法水解工艺研究

论文编号:HG187 论文字数:5401,页数:16

摘 要:为提高婴幼儿米粉的消化吸收性能和冲调性能,项目对传统的米粉生产技术与酶水解技术相结合进行了试验研究。采用α-淀粉酶AN30对大米淀粉水解,确定了米浆酶水解的主要工艺参数:在米浆自然pH值条件下,α-淀粉酶AN30最佳反应温度为60℃,酶添加量为0.05%~0.1%(按米粉质量百分比),水解30min,DE值达到5%~10%。另外,酶的钝化条件为:70℃,15min。试验发现,米浆在70℃~100℃的预糊化有助于提高α-淀粉酶的酶解率。没有经过酶解的产品消化指数为83.6%,而经过酶解后产品的消化指数为92.6%,酶解后的产品消化吸收率提高了10%左右,这将更有利于婴幼儿的消化吸收。

关键词:婴幼儿米粉;α-淀粉酶;水解;消化指数

Abstract: The digestion and absorption quality of infant formula rice flour could be improved if technology of enzymolysis of rice flour was combined with traditional production of infant formula rice flour. α-amylase named AN30 was selected to hydrolyze rice flour to meet dietary demand of infant. The results showed that the optimal enzymolysis temperature forα-amylase was 60℃ with crude pH value of rice slurry and DE value in the range of 5% to 10% was obtained by adding 0.05% to 0.1% enzyme amount to rice mass and hydrolyzing 30min. α-amylase AN30 was found to be inactive by heating at 70℃ for 15min. The ratio of enzymolysis was increased with pre-gelatinization of rice slurry at 70℃ to 100℃. Digest index of the product which has not been hydrolysis is 83.6%,and which has been hydrolysis is 92.6%,the digest index increased by 10%. This will be more conducive to digestion and absorption of infants formula.

Key words: infant formula rice flour;α-amylase; hydrolysis; digestion index

目 录

中文摘要I

英文摘要II

目录III

1. 绪论1

2. 实验部分3

2.1材料与仪器 3

2.1.1材料 3

2.1.2仪器 3

2.2实验方法3

2.2.1婴幼儿谷基配方米粉酶法生产工艺 3

2.2.2酶活性测定方法 3

2.2.3DE值测量方法 4

2.2.4粘度的测定方法 5

2.2.5产品消化吸收率测定 5

3. 结果与讨论6

3.1酶活性影响因素 6

3.2预糊化对酶解作用的影响 7

3.3酶添加量的确定 7

3.4酶钝化参数测定 8

3.5产品消化吸收率 9

4.总结与展望11

致谢12

参考文献13

婴幼儿谷基配方米粉酶法水解工艺研究......