响应面法优化橘核中柠檬苦素提取分离工艺研究

论文编号:HG130 论文字数:12520,页数:36

摘要：为优化柠檬苦素提取工艺，以1g桔核粉提取的柠檬苦素产量为指标，对料液比、超声时间、超声温度、超声功率、提取剂种类及乙醇浓度等因素进行了考察。在单因素实验的基础上，选取超声温度、超声时间和料液比为随机因子，利用响应面法对提取柠檬苦素的工艺进行优化，根据中心组合设计原理采用三因素三水平的响应面分析法，依据回归分析确定最优提取工艺条件。结果表明，柠檬苦素提取的最佳工艺条件为：超声温度为45.65℃，超声时间为17.77min，料液比为1：65.52。采用该工艺条件，1g桔核粉柠檬苦素类似物总产量可达到44.17mg。验证实验值为46.40mg，与理论值相对误差为4.8%。

关键词：柠檬苦素；超声；提取；响应面分析法；工艺优化

Abstracts: Limonin production from orange nuclear powder was carried out using ethanol with the assistance of ultrasonic. Experimental design was used to investigate the effect of three parameters (extraction temperature, extraction time, and ratio of ethanol to raw material) on limonin yields. The ranges of the factors investigated were 30~50℃ for extraction temperature (X1), 20-30min for extraction time (X2), and 50~70 for ratio of ethanol to raw material (X3). The central composite design showed that polynomial regression models were in good agreement with the experimental results with the coefficients of determination of 0.9900 for limonin yield. The optimal condition for limonin yield within the experimental range of the variables studied was at 17.8 min, 45.6℃, and 1:65.5. At this condition, the predicted yield of limonin extracted was 46.4mg.

Key words: Limonin；Ultrasound；Extraction；Response Surface Methodology；Optimization

目 录

中文摘要I

英文摘要II

目 录III

1 绪论1

1.1 柠檬苦素的来源1

1.2 柠檬苦素类似物及其配糖体的结构1

1.3 柠檬苦素类似物的理化性质2

1.4 柠檬苦素类似物的分布、含量及生物转化过程2

1.5 柠檬苦素类似物的生物活性功能4

1.6 柠檬苦素类似物的提取技术5

1.7 柠檬苦素类似物的分离纯化技术6

1.8 苦素类似物的检测技术6

1.9 柠檬苦素的应用8

1.10 柠檬苦素的发展前景9

2 实验部分11

2.1 实验原料、试剂与仪器11

2.2 实验步骤12

3 结果与讨论14

3.1 单因素实验结果与分析14

3.2 响应面法确立最佳工艺条件18

3.3 验证实验25

3.4 柠檬苦素的分离纯化25

4总结与展望27

4.1 总结27

4.2 展望27

致谢28

参考文献:29