茯苓多糖提取及氧化研究

论文编号:SW037论文字数:10290,页数:25

摘 要：以茯苓菌核为原料，在超声波辅助下，深讨了碱溶性茯苓多糖的提取条件。用2，2，6，6-四甲基哌啶-1-氧自由基（TEMPO）将茯苓多糖进行氧化，制得氧化茯苓多糖，用红外光谱进行了表征，并用胆酸结合能力的测定来比较多糖及氧化产物的降低胆固醇的能力,结果表明氧化后的茯苓多糖结合胆酸的能力比茯苓多糖下降。

关键词：茯苓多糖；提取；TEMPO；胆酸结合试验

Studies on the Extraction and Oxidation of Poria cocos Polysaccharides

Abstract: Alkali-soluble parchymaran was extracted by using dilute alkali (0.5mol/l NaOH) solution as a solvent with the assistance of ultrasonic and the extraction condition was examined. 2,2,6,6-Tetramethyl-1-piperidine oxoammonium ion (TEMPO)-mediated oxidation was applied to parchymaran. and the physicochemical properties of the resulting derivatives were investigated by using FT-IR and bile acid binding activity. The oxidized derivative showed lower bile acid binding activity than native glucan , it could be explained by the stronger interaction of carboxyl groups and bile acid .

Keywords: polysaccharides; extraction; ultrasonic wave; oxidation.

目 录

中文摘要I

英文摘要II

目录III

1、绪论1

1.1 茯苓的简介1

1.2 茯苓多糖的结构及作用1

1.3 茯苓多糖氧化后的作用2

1.4 茯苓多糖的提取2

1.5 粗多糖的纯化5

1.6 茯苓多糖的氧化5

1.7 茯苓多糖及其衍生物体外生物活性的检测6

1.8 实验思路6

2、实验部分7

2. 材料试剂与设备7

2.2 实验步骤7

3 结果与讨论11

3.1 波长的选择11

3.2 标准曲线的制作11

3.3 单因素分析12

3.4 茯苓多糖及其氧化衍生物的红外谱图15

3.5 胆酸结合能力试验17

4、结论18

4.1 结论18

4.2 展望18

致 谢19

参考文献20