甘薯抗氧化性及其黄酮含量的基因型差异研究

论文编号:SW096论文字数:14532,页数:29

摘 要：甘薯具有一定的营养价值和药用功能，它能消除活性氧，具有抗氧化能力。本文着重研究甘薯总抗氧化物质及黄酮类物质的提取和测定方法，进而分析基因型间的差异。由此了解其抗氧化的程度，帮助人们合理利用食品营养、研制能够预防癌症及心血管等方面疾病的功能性食品，还为甘薯黄酮类物质的开发和高黄酮高抗氧化性资源的筛选提供依据，促进农副产品的开发利用。实验表明，甘薯对自由基DPPH有很高的清除能力，所采用的21种基因型甘薯中，对DPPH的清除率最高达到97.3%，17种甘薯对其抑制率在89%以上，最低的为51.4%。采用以芦丁为标样的比色法对萧山，玉环两个试点的42种甘薯进行黄酮含量的测定，并采用近红外光谱分析技术得到另4个试点的84种甘薯中黄酮的含量，经分析表明黄酮含量存在显著的品中间和产地间的差异，紫883，徐55-2，金山1222 等品种稳定性强，受环境影响小，黄酮含量平均值都较高，是良好的选育品种。研究还表明甘薯抗氧化能力与黄酮含量之间为显著相关。

关键词： 抗氧化性；黄酮；近红外分析；基因型差异；甘薯

Study on genotypic variation of antioxidant activity

and flavonoid content in sweet potato storage roots

Abstract: Sweet potato has much physiological functionality, which contains rich of nutrition. It has antioxidant activity that can eliminate active oxygen. Extraction and mensuration of total antioxidant and flavonoid were studied in the paper, and genotypic variation was analyzed,which helped people to understand the degree of antioxidant, to reasonably use food nutrition and to develop research on the functional foods to prevent cancer and cardiovascular diseases. It also provides the basis for flavonoids exploiture and the high oxidation resistance and high-flavonoid resource selection, and promote the development and use of agricultural and sideline products. The result showed that sweetpotato had high ability to scavenge DPPH radical. Among 21 genotypes , the scavenging rate on DPPH radicals ranged from 51.4%-97.3%, about 17genotypes exceed 89%. The flavonoid content of sweetpotato has been measured by using Rutin as standard sample. As a result，there was variation in genotypic and location in the flavonoid contents, the Zi 883, Xu 55-2, Jinshan 1222 showed to be the choiceness genotypes, for they have high stabilization to location and high flavonoid content. During the experiment NRIS analysis had been used. The result also showed that the flavonoid content had significantly correlated to antioxidant ability.

Keywords：Antioxidant activity; Flavonoid; NIRS analysis; Genotypic variation;

Sweet potato

目 录

中文摘要I

英文摘要II

目 录III

1. 绪 论1

1.1引言1

1.2 甘薯抗氧化性研究3

1.3 本章小结7

2. 实验部分8

2.1 试剂、仪器与实验材料8

2.2 实验设计和样品处理10

2.3 本章小结12

3. 结果与讨论13

3.1 总抗氧化能力测定13

3.2 甘薯黄酮含量测定14

3.3 甘薯黄酮的NIRS分析17

3.4 甘薯抗氧化能力与黄酮含量的相关性研究20

3.5 本章小结21

4. 总结与展望22

致 谢23

参考文献24